CAN-ASC Webinar Series

A STEP-BY-STEP GUIDE TO GENERATING HIGH-PARAMETER FLOW CYTOMETRY DATA
Florian Mair, PhD 
FRIDAY, MARCH 26, 2021 12 pm PT, 3 pm ET

Florian MairFlorian Mair is an immunologist who brings Swiss precision to bear on his approach to flow cytometry. After completing his PhD at the University of Zurich, he moved to the Fred Hutchinson Cancer Research Center in Seattle where he is dissecting the function of human immune cells in non-lymphoid tissues during steady state and disease.  Florian is an ISAC Marylou scholar, an AAI Computational Intersect fellow, and has been actively engaged in teaching flow cytometry courses for several years, in particular systematic panel design and analysis of high-dimensional cytometry experiments.  In 2018, he published the first 30-parameter OMIP for the phenotyping of human dendritic cells (DCs). https://www.linkedin.com/in/florian-mair-fm/

Zoom Webinar with live Q&A, 45 min webinar, 45 min Q&A

BRING YOUR ENIGMAS, RIDDLES and CONUNDRUMS!

“With increasingly complex technology it is more important than ever to follow the principle that only high-quality experiments can yield high-quality data and relevant discoveries.”

WEBINAR DESCRIPTION 

space cartoonThe goal is to provide a practical guide for successful design of any complex panel from 10-40 (or more) parameters for conventional compensation-based as well as spectral cytometry. This includes:

  • Using the concept of spreading error (SE) for efficient panel design
  • A step-by-step approach to panel building
  • Standardization of flow data: considerations for longitudinal studies
  • Suggested controls for complex immunophenotyping experiments
  • Typical caveats: how to find and address compensation artifacts

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Webinar series registration: https://ubc.zoom.us/meeting/register/u5YudO6qrjMsH9cC9uoPfw7WRh7LR7uyxhZZ