Our lab is developing technology to solve the organ shortage problem. We work on different axes, including organ generation in xenogeneic species, xenogeneic immune-tolerance induction and long-term subzero organ preservation. Moreover, we study the mechanisms of cancer recurrence after liver transplantation and develop strategies to reduce such risk.
Impact of the presence of median arcuate ligament on biliary complications after liver transplantation
Eleftherios Gialamas and Michela Assalino and Laure Elkrief and Fani Apostolidou-Kiouti and Arthur Piveteau and Graziano Oldani and Philippe Compagnon and Thierry Berney
The Swiss approach to the COVID-19 outbreak
American Journal of Transplantation
Beat Moeckli and Andrea Peloso and Graziano Oldani and Lorenzo A. Orci and Vanessa Banz and Philipp Dutkowski and Christian Toso and Thierry Berney
Tolerogenic properties of liver macrophages in non-alcoholic steatohepatitis
Lorenzo A. Orci and Mario Kreutzfeldt and Nicolas Goossens and Laura Rubbia-Brandt and Florence Slits and Karim Hammad and Vaihere Delaune and Graziano Oldani and Francesco Negro and Sophie Clément and Carmen Gonelle-Gispert and Léo H. Buhler and Christian Toso and Stéphanie Lacotte
The impact of short-term machine perfusion on the risk of cancer recurrence after rat liver transplantation with donors after circulatory death
Graziano Oldani and Andrea Peloso and Florence Slits and Quentin Gex and Vaihere Delaune and Lorenzo A. Orci and Yohan van de Looij and Didier J. Colin and Stéphane Germain and Claudio de Vito and Laura Rubbia-Brandt and Stéphanie Lacotte and Christian Toso
Regenerative Medicine and Diabetes: Targeting the Extracellular Matrix Beyond the Stem Cell Approach and Encapsulation Technology.
Frontiers in endocrinology
Xenogeneic chimera—Generated by blastocyst complementation—As a potential unlimited source of recipient-tailored organs
Graziano Oldani and Andrea Peloso and Stéphanie Lacotte and Raphael Meier and Christian Toso
Immunosuppression-free transplantation of autologous livers grown in xenogeneic species
Creating autologous livers from patients’ cells would solve many of the important issues connected to transplantation, including immunosuppression-related side effects and the shortage of donor. Because autologous transplantable livers would be a rapidly available resource, we would see the expansion of transplant for oncologic indications and pediatric patients would receive a transplant long before becoming too sick or developing irreversible impairments. Overall, the way we treat chronic organ impairment and end stage disease would be revolutionized, with invaluable benefit for our patients, their families and the health care system.
The dramatic advances in induced pluripotent stem cells (iPSCs) technology over the last few years are turning the idea of xeno-grown autologous (x-gA) livers into a tangible reality. The advent of blastocyst complementation with iPSCs allowed for the generation of interspecific chimeras with few human cells in pigs and mice, and the creation of mouse complete pancreatic parenchyma in a rat. In addition, our group successfully transplanted chimeric mouse/rat livers into rats and showed improved function and survival over classic mouse to rat xenotransplantation. This model proves the ability for recipient-oriented remodeling and suggests that the liver can grow far beyond the size limits of the species of origin.
The present project aims to establish fundamental concepts which will set the stage for human-oriented studies and clinical applications. In particular:
1) Establish immunosuppression-free long- term survival of rats transplanted with autologous livers generated in mice
2) To generate xenogeneic autologous human livers in mice
Desensitization by clonal deletion, to accept future xenogeneic grafts: towards a “Transplant Vaccine”
Exposing a species to xenogenic tissue during the pre-immune phase (in-utero) may induce tolerance to xenogeneic solid organ transplantation in adulthood. We know from our experience that mouse-rat chimeras generated by blastocyst complementation are keen to accept organs and tissues from the two species. The aim of this project is to develop a strategy to expose mice to rat tissue in utero and to compare the outcomes of kidney and skin xenotransplantation to the matching whole-body chimeras.
Passive isochoric sub-zero organ preservation
Transplantable organs are usually stored at 0-1 degrees Celsius. Lower temperatures determine the creation of ice crystals that compromise cellular integrity. Being able to extend the time an organ can be stored by lowering the temperature and, at the same time avoiding ice formation would facilitate long-distance transportation. Moreover, it would allow the creation of an organ bank, although short-term. With that in mind, we are designing and testing passive isochoric chambers that may allow organ preservation at sub-zero temperatures.
Implantable devices to reduce complications of pancreatic surgery
The leak of the pancreatico-jejunal (PJ) anastomosis or the pancreatic stump is a common complication that afflicts patients resected of the pancreas. Such complication entails longer hospital stays in the best case, but can also start a chain of events, sometimes leading to the patient's death (1-2%). Our group has designed and patented an implantable device that may significantly reduce the leak rate of PJ anastomoses. We are now working on producing the device with absorbable polymers, aiming at a pilot study in the next 1-2 years.Grants
Swiss National Science Foundation (SNSF), Ambizione Grant. $1,050,000 CAD. August 2020 – July 2024
Innogap. $35,000 CAD. 2023
Fondation Schmieder – Bohrisch. $54,000 CAD. 2020
Société Académique de Genève. $27,000 CAD. 2020Honours & Awards
2022 Swiss Transplant Society Excellence Award
2019 Swiss Transplant Society Award: 1st prize for basic research
2017 Prix “Gilles Mentha” for translational transplantation research – Geneva University Hospitals
2012 SSCV Poster Price, Swiss Surgery Congress Annual Meeting – DavosResearch Group Members
Mina Kolahdouz, Research technician IV