- Overview
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Advances in mass spectrometry are the primary driving force behind the increasing number of inborn-errors of metabolism screened population-wide. The Newborn Screening laboratory at BC Children’s Hospital utilizes mass spectrometry for screening 45,000 newborns annually for a panel of 24 treatable disorders. Initial screening involves the analysis of dried blood spot cards by flow injection mass spectrometry. For a subset of conditions, abnormal screens are followed up with a second tier analysis using liquid chromatographic separations followed by mass spectrometry to increase analytical specificity and confirmation of diagnosis. My current research focuses on the development of novel mass spectrometry based assays for clinical diagnosis of inborn errors of metabolism.
Additional research interests include applications of mass spectrometry and other next generation analytical technologies for biomarker and drug target discovery. Investigation of inherited lipid metabolism disorders, lysosomal storage disorders, and environmental stress factors associated with lifetime cardiovascular disease risk are of particular interest.
- Publications
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Low LAL (Lysosomal Acid Lipase) Expression by Smooth Muscle Cells Relative to Macrophages as a Mechanism for Arterial Foam Cell Formation
Arteriosclerosis, Thrombosis, and Vascular Biology
Joshua A. Dubland and Sima Allahverdian and Katrina J. Besler and Carleena Ortega and Ying Wang and Collin S. Pryma and Kamel Boukais and Teddy Chan and Michael A. Seidman and Gordon A. Francis
DOI: 10.1161/ATVBAHA.120.316063
04/2021Role of inflammatory cytokines in genesis and treatment of atherosclerosis. Looking at foam cells through a different lens
Trends in Cardiovascular Medicine
Joshua A. Dubland
DOI: 10.1016/j.tcm.2021.02.008
03/2021Smooth Muscle Cells Contribute the Majority of Foam Cells in ApoE (Apolipoprotein E)-Deficient Mouse Atherosclerosis.
Arteriosclerosis, thrombosis, and vascular biology
DOI: 10.1161/atvbaha.119.312434
PubMed: 30786740
05/2019Pathways of smooth muscle foam cell formation in atherosclerosis.
Current opinion in lipidology
DOI: 10.1097/mol.0000000000000574
PubMed: 30664015
04/2019LAL (Lysosomal Acid Lipase) Promotes Reverse Cholesterol Transport In Vitro and In Vivo.
Arteriosclerosis, thrombosis, and vascular biology
DOI: 10.1161/ATVBAHA.117.310507
PubMed: 29599133
03/2018Randomized Controlled Trial for the Effect of Vitamin D Supplementation on Vascular Stiffness in CKD.
Clinical journal of the American Society of Nephrology : CJASN
DOI: 10.2215/CJN.10791016
PubMed: 28550081
05/2017Lamivudine, Entecavir, or Tenofovir Treatment of Hepatitis B Infection: Effects on Calcium, Phosphate, FGF23 and Indicators of Bone Metabolism.
Annals of hepatology
DOI: 10.5604/16652681.1231577
PubMed: 28233741
03/2017So Much Cholesterol: the unrecognized importance of smooth muscle cells in atherosclerotic foam cell formation.
Current opinion in lipidology
DOI: 10.1097/mol.0000000000000279
PubMed: 26836481
04/2016Lysosomal acid lipase: at the crossroads of normal and atherogenic cholesterol metabolism.
Frontiers in cell and developmental biology
DOI: 10.3389/fcell.2015.00003
PubMed: 25699256
02/2015Determination of serum aldosterone by liquid chromatography and tandem mass spectrometry: a liquid–liquid extraction method for the ABSCIEX API-5000 mass spectrometry system
Journal of Clinical Pathology
DOI: 10.1136/jclinpath-2011-200564
05/2012Carbon–carbon bond-forming reactions of a-carbonyl carbocations: exploration of a reversed-polarity equivalent of enolate chemistry
Tetrahedron
DOI: 10.1016/j.tet.2011.07.065
09/2011Serum-protein interactions with anticancer Ru(III) complexes KP1019 and KP418 characterized by EPR.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
DOI: 10.1007/s00775-009-0578-5
PubMed: 19707803
08/2009 - Research
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Grants
BCCHR Clinical Research Support Development Award; Next generation urine metabolic profiling for the diagnosis and monitoring of Inborn Errors of Metabolism, $25,000; Principal Investigator
Bojana Rakic; Co-Investigators
Joshua Dubland, Ramona Salvarinova-Zivkovic, Graham Sinclair
Honours & Awards2019 Young investigator educational grant from Mass Spectrometry
Applications to the Clinical Lab
2018 Arteriosclerosis, Thrombosis, & Vascular Biology travel award for young investigators
2018 CIHR travel award - Institute Community Support in partnership with Institute of Circulatory & Respiratory Health
2017 Canadian Lipoprotein Conference graduate student presentation award
2017 Canadian Society for Arteriosclerosis, Thrombosis & Vascular Biology travel award
2016 Canadian Lipoprotein Conference graduate student presentation award
New research paves way to better treatment for common childhood brain cancer
While chemotherapy and radiation are essential treatments for overcoming medulloblastoma , finding new ways to treat the disease more effectively could lessen the later-in-life impacts of treatment and ensure more children survive.
