Metabolic

Active-B12 (Holotranscobalamin) vitamin B12/cobalamin complex contains the biologically available form of B12

  • Abbott Architect Immunoanalyzer
  • Samples type:  human serum, other sample types should not be used

Vitamin B12 (Total) 

  • Abbott Architect immunoanalyzer
  • Sample type:  human plasma (lithium heparin, sodium heparin or dipotassium EDTA) or serum 

Methylmalonic acid (functional indicator of vitamin B12 status) 

  • HPLC MS/MS
  • Sample type: human serum or plasma (lithium heparin or EDTA), dried blood spots
  • Analyses includes 2-methylcitric acid and succinic acid

C-peptide  

  • Abbott Architect immunoanalyzer
  • Human peptide connecting A & B chains of insulin in the precursor molecule proinsulin
  • serum; heparin or EDTA plasma

Cortisol:

  • Abbott Architect immunoanalyzer
  • Major glucocorticoid hormone secreted by the adrenal cortex
  • Human serum; heparin or EDTA plasma, urine.

Ferritin:

  • Abbott Architect Immunoanalyzer

  • Iron binding protein found in human plasma/serum.  About 2/3 iron stores are found in ferritin

  • Sample type:  human samples only.

    • Serum is preferred

    • Tripotassium EDTA can be used although the values will be about 10% below those given by serum
    • Heparin can be used although the values will be higher than those given by serum

Folate:

  • Abbott Architect Immunoanalyzer
  • Sample type:  human serum or plasma (lithium heparin)

Red blood cell folate

  • Abbott Architect Immunoanalyzer
  • Sample type:  whole blood collected in vacutainers (dipotassium EDTA or tripotassium EDTA)

Reduced folate forms and unmetabolized folic acid

  • HPLC MSMS (externally validated assay)
  • Sample type: human serum or plasma (lithium heparin), whole blood collected in vacutainers (heparin), washed red blood cells

Hemoglobin A1c (Monitoring)

  • Abbott Architect Immunoanalyzer
  • Whole blood collected into EDTA tubes.  Do not use heparin with this assay.

Homocysteine

  • Abbott Architect Immunoanalyzer
  • Sample type:  human serum or plasma 

Insulin 

  • Abbott Architect Immunoanalyzer
  • Sample type:  human serum; heparin or EDTA plasma

Intact PTH   Intact parathyroid hormone

  • Abbott Architect Immunoanalyzer
  • Serum, heparin or potassium EDTA plasma

Vitamin D

  • Abbott Architect Immunoanalyzer for 25-hydroxy vitamin D3 only.  Serum, heparin or EDTA plasma 
  • HPLC-MSMS.  Cholecalciferol (non-hydroxylated vitamin D3), 25 hydroxy vitamin D3, 24,25 dihydroxyvitamin D3.  Serum or plasma.

Lutein

  • HPLC photodiode array detection
  • Serum or plasma 

Pyroglutamic acid

  • HPLC MSMS
  • Sample type:  serum or plasma (animal or human)
  • Volume serum/plasma required:  25-50ul

Methionine

  • HPLC MSMS
  • Sample type:  serum or plasma (animal or human)
  • Volume serum/plasma required:  25-50ul
  • Analyses include cysteine and homocysteine

Cysteine

  • HPLC MSMS
  • Sample type:  serum or plasma (animal or human)
  • Volume serum/plasma required:  25-50ul
  • Analyses include homocysteine and methionine

Choline

  • HPLC MSMS
  • Sample type:  serum or plasma (animal or human), tissues, milk 
  • Volume serum/plasma required:  25-50ul
  • Analyses include betaine and dimethylglycine

Betaine

  • HPLC MSMS
  • Sample type:  serum or plasma (animal or human), tissues, milk 
  • Volume serum/plasma required:  25-50ul
  • Analyses include choline and dimethylglycine

Dimethylglycine

  • HPLC MSMS
  • Sample type:  serum or plasma (animal or human), tissues, 
  • Analyses include choline and betaine

Trimethylamine

  • HPLC MSMS
  • Sample type:  serum/plasma or urine  (animal or human)
  • Analyses includes trimethylamine oxide

Trimethylamine oxide

  • HPLC MSMS
  • Sample type:  serum/plasma or urine (animal or human)
  • Analyses includes trimethylamine

S-adenosyl methionine and S-adenosyl homocysteine

  • HPLC MSMS
  • Samples type:  serum/plasma, tissues (must be specially prepared)

Taurine

  • HPLC MS/MS
  • Sample type:  serum or plasma (animal or human), tissues
  • Analyses include choline, betaine, dimethylglycine, methionine, homocysteine, serine, glycine, cystathionine, cysteine

Vitamin B6 (pyridoxal 5’-phosphate, pyridoxal, pyridoxic acid)

  • HPLC MS/MS
  • Sample type:  serum or plasma (animal or human)
  • Analyses include riboflavin, FMN, FAD, tryptophan, kynurenine metabolites

Vitamin B2 (riboflavin, FMN, FAD)

  • HPLC MS/MS
  • Sample type:  serum or plasma (animal or human)
  • Analyses include pyridoxal 5’-phosphate, pyridoxal, pyridoxic acid, tryptophan, kynurenine metabolites

Tryptophan and kynurenine metabolites (…)

  • HPLC MS/MS
  • Sample type:  serum or plasma (animal or human)
  • Analyses include pyridoxal 5’-phosphate, pyridoxal, pyridoxic acid, riboflavin, FMN, FAD